Synopsis
The BioSAXS beamline is a highly automated beamline dedicated to the study of proteins, macromolecular complexes, viruses etc., in solution. Samples can be investigated under various conditions (temperature, buffer, pH, kinetics) in a high-throughput manner or a HPLC unit can be used for in-situ (online) purification.
Status:
open
Disciplines
- Life Sciences
- Chemistry
- Medicine
Applications
- Structural biology
- Pharmaceuticals
Techniques
-
BioSAXS - small-angle X-ray scattering (proteins/DNA)
-
SAXS - small-angle X-ray scattering
Beam size
- Minimum (H x V) : 50.0
x 50.0
µm²
-
Maximum (H x V) : 2.0
x 1.0
mm²
Sample environments
- Quartz capillary as a part of automated sample changer allowing temperature variations (from 4 to 60°C)
- HPLC (high performance liquid chromatography) system can be used in parallel with sample changer
Detectors
Pernot P., Theveneau P., Giraud T., Nogueira Fernandes R., Nurizzo D., Spruce D., Surr J., McSweeney S., Round A., Felisaz F., Foedinger L., Gobbo A., Huet J., Villard C. and Cipriani F., "New beamline dedicated to solution scattering from biological macromolecules at the ESRF", Journal of Physics: Conference Series 247 (2010) 012009-1-012009-8.
A non-catalytic herpesviral protein reconfigures ERK-RSK signaling by targeting kinase docking systems in the host
Alexa A., Sok P., Gross F., Albert K., Kobori E., Póti A.L., Gógl G., Bento I., Kuang E., Taylor S.S., Zhu F., Ciliberto A., Reményi A.,
Nature Communications 13, 472-1-472-19 (2022)
The intrinsically disordered SARS-CoV-2 nucleoprotein in dynamic complex with its viral partner nsp3a
Bessa L.M., Guseva S., Camacho-Zarco A.R., Salvi N., Maurin D., Mariño Pérez L., Botova M., Malki A., Nanao M., Ringkjøbing Jensen M., Ruigrok R.W.H., Blackledge M.,
Science Advances 8, eabm4034-1-eabm4034-12 (2022)
Structural dynamics of the C-terminal X domain of Nipah and Hendra viruses controls the attachment to the C-terminal tail of the nucleocapsid protein
Bourhis J.M., Yabukarski F., Communie G., Schneider R., Volchkova V.A., Frénéat M., Gérard F.C., Ducournau C., Mas C., Tarbouriech N., Ringkjøbing Jensen M., Volchkov V.E., Blackledge M., Jamin M.,
Journal of Molecular Biology 434, 167551-1-167551-28 (2022)
Amyloid and hydrogel formation of a peptide sequence from a coronavirus spike protein
Castelletto V., Hamley I.W.,
ACS Nano 16, 1857-1867 (2022)
Biochemical and biophysical features of disease-associated tau mutants V363A and V363I
De Luigi A., Colombo L., Russo L., Ricci C., Bastone A., Cimini S., Tagliavini F., Rossi G., Cantù L., Del Favero E., Salmona M.,
Biochimica et Biophysica Acta (BBA) - Proteins & Proteomics 1870, 140755-1-140755-11 (2022)
Extracellular endosulfatase Sulf-2 harbors a chondroitin/dermatan sulfate chain that modulates its enzyme activity
El Masri R., Seffouh A., Roelants C., Seffouh I., Gout E., Pérard J., Dalonneau F., Nishitsuji K., Noborn F., Nikpour M., Larson G., Crétinon Y., Friedel-Arboleas M., Uchimura K., Daniel R., Lortat-Jacob H., Filhol O., Vivès R.R.,
Cell Reports 38, 110516-1-110516-19 (2022)