Synopsis
The BioSAXS beamline is a highly automated beamline dedicated to the study of proteins, macromolecular complexes, viruses etc., in solution. Samples can be investigated under various conditions (temperature, buffer, pH, kinetics) in a high-throughput manner or a HPLC unit can be used for in-situ (online) purification.
Status:
open
Disciplines
- Life Sciences
- Chemistry
- Medicine
Applications
- Structural biology
- Pharmaceuticals
Techniques
-
BioSAXS - small-angle X-ray scattering (proteins/DNA)
-
SAXS - small-angle X-ray scattering
Beam size
- Minimum (H x V) : 50.0
x 50.0
µm²
-
Maximum (H x V) : 2.0
x 1.0
mm²
Sample environments
- Quartz capillary as a part of automated sample changer allowing temperature variations (from 4 to 60°C)
- HPLC (high performance liquid chromatography) system can be used in parallel with sample changer
Detectors
Pernot P., Theveneau P., Giraud T., Nogueira Fernandes R., Nurizzo D., Spruce D., Surr J., McSweeney S., Round A., Felisaz F., Foedinger L., Gobbo A., Huet J., Villard C. and Cipriani F., "New beamline dedicated to solution scattering from biological macromolecules at the ESRF", Journal of Physics: Conference Series 247 (2010) 012009-1-012009-8.
Phospholipase D inhibitors screening: Probing and evaluation of ancient and novel molecules
Arhab Y., Bessaa K., Abla H., Aydin M., Rahier R., Comte A., Brizuela L., Mebarek S., Perret F., Cherrier M.V., Abousalham A., Noiriel A.,
International Journal of Biological Macromolecules 166, 1131-1140 (2021)
Small-angle X-ray scattering experiments of monodisperse intrinsically disordered protein samples close to the solubility limit
Martin E.W., Hopkins J.B., Mittag T.,
Methods in Enzymology 646, 185-222 (2021)
Beyond structural models for the mode of action: How natural antimicrobial peptides affect lipid transport
Nielsen J., Bjornestad V., Pipich V., Jenssen H., Lund R.,
Journal of Colloid and Interface Science 582, 793-802 (2021)
Inference of molecular structure for characterization and improvement of clinical grade immunocytokines
Ongaro T., Guarino S.R., Scietti L., Palamini M., Wulhfard S., Neri D., Villa A., Forneris F.,
Journal of Structural Biology 213, 107696-1-107696-10 (2021)
A structure-function study of ZraP and ZraS provides new insights into the two-component system Zra
Taher R., de Rosny E.,
Biochimica et Biophysica Acta (BBA) - General Subjects 1865, 129810-1-129810-9 (2021)
K+ vs. Na+ effects on the self-assembly of guanosine 5′-monophosphate: A solution SAXS structural study
Baldassarri E.J., Ortore M.G., Spinozzi F., Round A., Ferrero C., Mariani P.,
Nanomaterials 10, 629-1-629-20 (2020)