ID30B / MAD

Synopsis

ID30B is a highly automated, undulator-based, tunable beamline dedicated to macromolecular crystallography.

Disciplines

• Life Sciences

Applications

• Life sciences
• Biological Science
• Biochemistry
• Structural biology

Techniques

• MAD - multi-wavelength anomalous dispersion
• MX - macromolecular crystallography

Sample environments

• ID30B is equipped with a modified microdiffractometer (MD2-S), allowing data collection from micron-sized crystals mounted on conventional cryo-supports or in crystallisation plates (in situ).
• A new generation, flexible and high throughput sample changer (FlexHCD) capable of transferring samples from either EMBL/ESRF pucks or Unipucks has also been developed for ID30B in a collaboration between the ESRF and EMBL-Grenoble.

Detectors

• PILATUS3 6M 1000 µm Si sensor (Dectris). This detector was funded with support from the French Infrastructure for Integrated Structural Biology (FRISBI) ANR-10-INBS-05 to the CNRS and the ESRF.
• AXAS-A X-ray fluorescence detector (KETEK)

Technical references

McCarthy, A. A., Barrett, R., Beteva, A., Caserotto, H., Dobias, F., Felisaz, F., Giraud, T., Guijarro, M., Janocha, R., Khadrouche, A., Lentini, M., Leonard, G. A., Lopez Marrero, M., Malbet-Monaco, S., McSweeney, S., Nurizzo, D., Papp, G., Rossi, C., Sinoir, J., Sorez, C., Surr, J., Svensson, O., Zander, U., Cipriani, F., Theveneau, P., Mueller-Dieckmann, C. ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF. J. Synchrotron Radiat. 25,1249-1260 (2018).

Mueller-Dieckmann, C., Bowler, M. W., Carpentier, P., Flot, D., McCarthy, A. A., Nanao, M. H., Nurizzo, D., Pernot, P., Popov, A., Round, A., Royant, A., de Sanctis, D., von Stetten, D., Leonard, G. A. The status of the macromolecular crystallography beamlines at the European Synchrotron Radiation Facility, Eur. Phys. J. Plus 130, 70 (2015).