Synopsis
The BioSAXS beamline is a highly automated beamline dedicated to the study of proteins, macromolecular complexes, viruses etc., in solution. Samples can be investigated under various conditions (temperature, buffer, pH, kinetics) in a high-throughput manner or a HPLC unit can be used for in-situ (online) purification.
Status:
open
Disciplines
- Life Sciences
- Chemistry
- Medicine
Applications
- Structural biology
- Pharmaceuticals
Techniques
-
BioSAXS - small-angle X-ray scattering (proteins/DNA)
-
SAXS - small-angle X-ray scattering
Beam size
- Minimum (H x V) : 50.0
x 50.0
µm²
-
Maximum (H x V) : 2.0
x 1.0
mm²
Sample environments
- Quartz capillary as a part of automated sample changer allowing temperature variations (from 4 to 60°C)
- HPLC (high performance liquid chromatography) system can be used in parallel with sample changer
Detectors
Pernot P., Theveneau P., Giraud T., Nogueira Fernandes R., Nurizzo D., Spruce D., Surr J., McSweeney S., Round A., Felisaz F., Foedinger L., Gobbo A., Huet J., Villard C. and Cipriani F., "New beamline dedicated to solution scattering from biological macromolecules at the ESRF", Journal of Physics: Conference Series 247 (2010) 012009-1-012009-8.
An Interdisciplinary Approach Provides Insights into the Pronounced Selectivity of Compound 42 for DPP9
Beyens O., Corthaut S., Lambeir A., Van der Veken P., Sterckx Yann G.-J., De Meester I., De Winter H.,
ChemMedChem 20, e202400700-1-e202400700-12 (2025)
Rabies virus phosphoprotein exhibits thermoresponsive phase separation with a lower critical solution temperature
Bouchama F., Mubashira K., Mas C., Le Roy A., Ebel C., Bourhis J., Zemb T., Prévost S., Jamin M.,
Journal of Molecular Biology 437, 168889-1-168889-25 (2025)
Time-resolved X-ray solution scattering unveils the events leading to hemoglobin heme capture by staphylococcal IsdB
De Bei O., Marchetti M., Guglielmo S., Gianquinto E., Spyrakis F., Campanini B., Bettati S., Levantino M., Ronda L.,
Nature Communications 16, 1361-1-1361- (2025)
An albumin unfolding and refolding cycle induced by a time-controlled pH jump
Del Giudice A., Del Giudice D., Spatola E., Alemanno V., Galantini L., Di Stefano S.,
Organic & Biomolecular Chemistry 23, 118-125 (2025)
The structure of FCGBP is formed as a disulfide‐mediated homodimer between its C‐terminal domains
Ehrencrona E., Gallego P., Trillo-Muyo S., García-Bonete M.J., Recktenwald C.V., Hansson G.C., Johansson M.E.V.,
FEBS Journal 292, 582-601 (2025)
Enhancing pesticide detection: The role of serine in lipopeptide nanostructures and their self-assembly dynamics
Gerbelli B.B., Sodré P.T., Filho P.L.O., Coutinho-Neto M.D., Hamley I.W., Seitsonen J., Alves W.A.,
Journal of Colloid and Interface Science 690, 137271-1-137271-14 (2025)
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