BM29: BioSAXS - small-angle scattering for biological samples

Synopsis

The BioSAXS beamline is a highly automated beamline dedicated to the study of proteins, macromolecular complexes, viruses etc., in solution. Samples can be investigated under various conditions (temperature, buffer, pH, kinetics) in a high-throughput manner or a HPLC unit can be used for in-situ (online) purification.

Status: open

Disciplines

Life Sciences
Chemistry
Medicine

Applications

Structural biology
Pharmaceuticals

Techniques

BioSAXS - small-angle X-ray scattering (proteins/DNA)
SAXS - small-angle X-ray scattering

Energy range

7.0 - 15.0 keV

Beam size

Minimum (H x V) : 50.0 x 50.0 µm²
Maximum (H x V) : 2.0 x 1.0 mm²

Sample environments

Quartz capillary as a part of automated sample changer allowing temperature variations (from 4 to 60°C)
HPLC (high performance liquid chromatography) system can be used in parallel with sample changer

Detectors

Pilatus3 2M in-vac

Pernot P., Theveneau P., Giraud T., Nogueira Fernandes R., Nurizzo D., Spruce D., Surr J., McSweeney S., Round A., Felisaz F., Foedinger L., Gobbo A., Huet J., Villard C. and Cipriani F., "New beamline dedicated to solution scattering from biological macromolecules at the ESRF", Journal of Physics: Conference Series 247 (2010) 012009-1-012009-8.

Latest publications

View all publications

Toward nanodisc tailoring for SANS study of membrane proteins

Batchu K.C., Tully M.D., Martel A.,
Bioengineering 13, 87-1-87-15 (2026)

Conformational flexibility and transient structure of the proline-rich domain in p53

Berggren A., Bakker M., Fisher H., Skepö M.,
Biophysical Journal 125, 1914-1925 (2026)

Ligands reshape the compactness, stability, and topology of telomeric G-quadruplex dimers

Bertini L., Libera V., Arciuolo V., Trapella M., Marzano S., Mostarac D., Schirò G., Petrillo C., Giancola C., De Michele C., Amato J., Comez L., Pagano B., Paciaroni A.,
Nucleic Acids Research 54, gkag403-1-gkag403-14 (2026)

Computational enzyme design by catalytic motif scaffolding

Braun M., Tripp A., Chakatok M., Kaltenbrunner S., Fischer C., Stoll D., Bijelic A., Elaily W., Totaro M.G., Moser M., Hoch S.Y., Lechner H., Rossi F., Aleotti M., Hall M., Oberdorfer G.,
Nature 649, 237-245 (2026)

Consequences of encapsulated vs. covalently attached Pd-NHC amphiphilic polymeric nanocatalysts in bioorthogonal catalysis

de Jonge L., Hazenberg I., Singh H., Loman T., Prakasham A.P., Palmans A.R.A.,
Chemical Communications 62, 4094-4098 (2026)

On the self-assembly of porphyrins and bile salts: The supramolecular chiral induction by steroidal surfactants

D’Annibale V., Piccirillo L., Del Giudice A., Mezzetti L., Bassani D.M., Raffy G., Pouget E., Oda R., Giustini M., D’Abramo M., Schillen K., Monti D., Galantini L.,
Colloids and Surfaces A 747, 140900-1-140900-15 (2026)

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