BM29: BioSAXS - small-angle scattering for biological samples

Synopsis

The BioSAXS beamline is a highly automated beamline dedicated to the study of proteins, macromolecular complexes, viruses etc., in solution. Samples can be investigated under various conditions (temperature, buffer, pH, kinetics) in a high-throughput manner or a HPLC unit can be used for in-situ (online) purification.

Status: open

Disciplines

Life Sciences
Chemistry
Medicine

Applications

Structural biology
Pharmaceuticals

Techniques

BioSAXS - small-angle X-ray scattering (proteins/DNA)
SAXS - small-angle X-ray scattering

Energy range

7.0 - 15.0 keV

Beam size

Minimum (H x V) : 50.0 x 50.0 µm²
Maximum (H x V) : 2.0 x 1.0 mm²

Sample environments

Quartz capillary as a part of automated sample changer allowing temperature variations (from 4 to 60°C)
HPLC (high performance liquid chromatography) system can be used in parallel with sample changer

Detectors

Pilatus3 2M in-vac

Pernot P., Theveneau P., Giraud T., Nogueira Fernandes R., Nurizzo D., Spruce D., Surr J., McSweeney S., Round A., Felisaz F., Foedinger L., Gobbo A., Huet J., Villard C. and Cipriani F., "New beamline dedicated to solution scattering from biological macromolecules at the ESRF", Journal of Physics: Conference Series 247 (2010) 012009-1-012009-8.

Latest publications

View all publications

Toward nanodisc tailoring for SANS study of membrane proteins

Batchu K.C., Tully M.D., Martel A.,
Bioengineering 13, 87-1-87-15 (2026)

Studies of the structural properties and stability of the molybdenum transport protein ModA from Oleidesulfovibrio alaskensis G20 upon metal binding

Rivas M.G., Correia Cordeiro R.S., Nair R.R., Albuquerque L.J.C., Correia M.A.S., Panjkovich A., Svergun D.I., Santos-Silva T.,
Archives of Biochemistry and Biophysics 775, 110642-1-110642-11 (2026)

Dissection of an anaerobic fungal cellulosomal endoglucanase: Impact of the dockerin module on activity, thermostability, and flexibility

Andrade V.B., Tramontina R., Almeida D.V., Tomazetto G., da Silva V.M., Gabel F., Marcello Y.M.B., Scott A.L., Squina F.M., Garcia W.,
ACS Omega 10, 20474-20486 (2025)

HP1 loses its chromatin clustering and phase separation function across evolution

Bensaha S., Lewandowska D., Muzzopappa F., Hutin S., Tully M.D., Anfossi M., Cammas F.M., Normand C., Erdel F.,
Nature Communications 16, 6375-1-6375-15 (2025)

An interdisciplinary approach provides insights into the pronounced selectivity of compound 42 for DPP9

Beyens O., Corthaut S., Lambeir A., Van der Veken P., Sterckx Y.G.J., De Meester I., De Winter H.,
ChemMedChem 20, e202400700-1-e202400700-12 (2025)

Capabilities of SEC-SAXS method in research of polydisperse systems: The case of apoferritin

Bezruchko I.O., Kuklina D.D., Dronova E.A., Kazantsev A.S., Khokhlov A.A., Vlasov A.V., Kuklin A.I., Ryzhykau Y.L.,
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology 19, 20-26 (2025)

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