Synopsis
Fixed energy microfocus beamline
Status:
open
Disciplines
Applications
- MASSIF-3 is a microfocus beamline (15 µm diameter), operating at fixed energy (12.81 keV). The beamline is dedicated to protein crystals of small size, inhomogeneous protein crystals, and fast data collection, and is also suitable for SAD measurements (Se, Pt, Au,...). MASSIF-3 is equipped with a MD2 micro-diffractometer and with a FlexHCD sample changer for SPINE pucks and UniPucks. Diffraction data are recorded on a Eiger X 4M detector that allows data collection at a very high frame rate (up to 750 Hz).
Techniques
-
Anomalous diffraction
-
MX - macromolecular crystallography
-
Microbeam
-
Microcrystallography
-
Optical spectroscopy
-
Single-crystal diffraction
-
XRD - X-ray diffraction
Beam size
- Minimum (H x V) : 15.0
x 15.0
µm²
-
Maximum (H x V) : 15.0
x 15.0
µm²
Sample environments
- 100 K cryostream
- automatic sample changer
- dehydration device
- online microspec
Detectors
References:
ID30A-3 (MASSIF-3) – a beamline for macromolecular crystallography at the ESRF with a small intense beam, D. Von Stetten et al., J. Synchrotron Radiat. (2020) 27: 844–851. doi: 10.1107/S1600577520004002
The Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation, P. Theveneau et al. (2013) J. Phys.: Conf. Ser. 425 012001 doi:10.1088/1742-6596/425/1/012001
Unveiling molecular details behind improved activity at neutral to alkaline pH of an engineered DyP-type peroxidase
Borges P.T., Silva D., Silva T.F.D., Brissos V., Cañellas M., Lucas M.F., Masgrau L., Melo E.P., Machuqueiro M., Frazão C., Martins L.O.,
Computational and Structural Biotechnology Journal 20, 3899-3910 (2022)
Structure of the DNMT3B ADD domain suggests the absence of a DNMT3A-like autoinhibitory mechanism
Boyko K., Arkova O., Nikolaeva A., Popov V.O., Georgiev P., Bonchuk A.,
Biochemical and Biophysical Research Communications 619, 124-129 (2022)
New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain
Chakraborty S., Alam B., Biswas S.,
Biochemical and Biophysical Research Communications 590, 145-151 (2022)
Structural basis of human dimeric alpha-amino-beta-carboxymuconate-epsilon-semialdehyde decarboxylase inhibition with TES-1025
Cianci M., Giacchè N., Cialabrini L., Carotti A., Liscio P., Rosatelli E., De Franco F., Gasparrini M., Robertson J., Amici A., Raffaelli N., Pellicciari R.,
Frontiers in Molecular Biosciences 9, 834700-1-834700-9 (2022)
Inhibition of SRP-dependent protein secretion by the bacterial alarmone (p)ppGpp
Czech L., Mais C.N., Kratzat H., Sarmah P., Giammarinaro P., Freibert S.A., Esser H.F., Musial J., Berninghausen O., Steinchen W., Beckmann R., Koch H.G., Bange G.,
Nature Communications 13, 1069-1-1069-14 (2022)
Crystal structure of the kringle domain of human receptor tyrosine kinase-like orphan receptor 1 (hROR1)
Guarino S.R., Di Bello A., Palamini M., Capillo M.C., Forneris F.,
Acta Crystallographica F 78, 185-192 (2022)