ID30A-3 / MASSIF-3

Applications

MASSIF-3 is a microfocus beamline (15 µm diameter), operating at fixed energy (12.81 keV). The beamline is dedicated to protein crystals of small size, inhomogeneous protein crystals, and fast data collection, and is also suitable for SAD measurements (Se, Pt, Au,...). MASSIF-3 is equipped with a MD2 micro-diffractometer and with a FlexHCD sample changer for SPINE pucks and UniPucks. Diffraction data are recorded on a Eiger X 4M detector that allows data collection at a very high frame rate (up to 750 Hz).

Techniques

Anomalous diffraction

MX - macromolecular crystallography

Microbeam

Microcrystallography

Optical spectroscopy

Single-crystal diffraction

XRD - X-ray diffraction

Energy range

12.81 - 12.81 keV

Beam size

Minimum (H x V) : 15.0 x 15.0 µm²
Maximum (H x V) : 15.0 x 15.0 µm²

Detectors

Eiger X 4M

References:

ID30A-3 (MASSIF-3) – a beamline for macromolecular crystallography at the ESRF with a small intense beam, D. Von Stetten et al., J. Synchrotron Radiat. (2020) 27: 844–851. doi: 10.1107/S1600577520004002

The Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation, P. Theveneau et al. (2013) J. Phys.: Conf. Ser. 425 012001 doi:10.1088/1742-6596/425/1/012001

Rational control of off-state heterogeneity in a photoswitchable fluorescent protein provides switching contrast enhancement

Adam V., Hadjidemetriou K., Jensen N., Shoeman R.L., Woodhouse J., Aquila A., Banneville A.S., Barends T.R.M., Bezchastnov V., Boutet S., Byrdin M., Cammarata M., Carbajo S., Eleni Christou N., Coquelle N., de la Mora E., El Khatib M., Moreno Chicano T., Bruce Doak R., Fieschi F., Foucar L., Glushonkov O., Gorel A., Grünbein M.L., Hilpert M., Hunter M., Kloos M., Koglin J.E., Lane T.J., Liang M., Mantovanelli A., Nass K., Nass Kovacs G., Owada S., Roome C.M., Schirò G., Seaberg M., Stricker M., Thépaut M., Tono K., Ueda K., Uriarte L.M., You D., Zala N., Domratcheva T., Jakobs S., Sliwa M., Schlichting I., Colletier J.P., Bourgeois D., Weik M.,
ChemPhysChem 23, e202200192-1-e202200192-11 (2022)

Slow protein dynamics probed by time-resolved oscillation crystallography at room temperature

Aumonier S., Engilberge S., Caramello N., von Stetten D., Gotthard G., Leonard G.A., Mueller-Dieckmann C., Royant A.,
IUCrJ 9, 756-767 (2022)

Tertiary and quaternary structure organization in GMP synthetases: Implications for catalysis

Ballut L., Violot S., Galisson F., Gonçalves I.R., Martin J., Shivakumaraswamy S., Carrique L., Balaram H., Aghajari N.,
Biomolecules 12, 871-1-871-15 (2022)

Unveiling molecular details behind improved activity at neutral to alkaline pH of an engineered DyP-type peroxidase

Borges P.T., Silva D., Silva T.F.D., Brissos V., Cañellas M., Lucas M.F., Masgrau L., Melo E.P., Machuqueiro M., Frazão C., Martins L.O.,
Computational and Structural Biotechnology Journal 20, 3899-3910 (2022)

Structure of the DNMT3B ADD domain suggests the absence of a DNMT3A-like autoinhibitory mechanism

Boyko K., Arkova O., Nikolaeva A., Popov V.O., Georgiev P., Bonchuk A.,
Biochemical and Biophysical Research Communications 619, 124-129 (2022)

New insights of falcipain 2 structure from Plasmodium falciparum 3D7 strain

Chakraborty S., Alam B., Biswas S.,
Biochemical and Biophysical Research Communications 590, 145-151 (2022)

ID30A-3 / MASSIF-3

Synopsis

Disciplines

Applications

Techniques

Energy range

Beam size

Sample environments

Detectors

Latest publications