ID30A-3 / MASSIF-3

Synopsis

MASSIF-3 is a minifocus beamline (15 µm diameter), operating at fixed energy (12.81 keV). The beamline is dedicated to protein crystals of small size, inhomogeneous protein crystals, and fast data collection, and is also suitable for SAD measurements (Se, Pt, Au,...). MASSIF-3 is equipped with a MD2 micro-diffractometer and with a FlexHCD sample changer for SPINE pucks and UniPucks. Diffraction data are recorded on an Eiger1 X 4M detector that allows data collection at a very high frame rate (up to 750 Hz). The high resolution limit on the beamline is 1.17 Å.

Status: open

Disciplines

Medicine
Life Sciences
Chemistry
Environmental Sciences

Applications

Macromolecular crystallography
Time resolved serial oscillation crystallography (SOX)

Techniques

Anomalous diffraction
MX - macromolecular crystallography
Microbeam
Microcrystallography
Optical spectroscopy
Single-crystal diffraction
XRD - X-ray diffraction

Energy range

12.81 keV

Beam size

Minimum (H x V) : 15.0 x 15.0 µm²
Maximum (H x V) : 15.0 x 15.0 µm²

Sample environments

100 K cryostream
automatic sample changer
dehydration device
online microspec

Detectors

Eiger1 X 4M

Technical details

High resolution limit - 1.17Å

References:

ID30A-3 (MASSIF-3) – a beamline for macromolecular crystallography at the ESRF with a small intense beam, D. Von Stetten et al., J. Synchrotron Radiat. (2020) 27: 844–851. doi: 10.1107/S1600577520004002

The Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation, P. Theveneau et al. (2013) J. Phys.: Conf. Ser. 425 012001 doi:10.1088/1742-6596/425/1/012001

Latest publications

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The mechanism of pathogenic α1-antitrypsin aggregation in the human liver

Aldobiyan I., Elliston E.L.K., Heyer-Chauhan N., Arold S.T., Zhao L., Huntington B., Lowen S.M., Orlova E.V., Irving J.A., Lomas D.A.,
Proceedings of the National Academy of Sciences of the USA 122, e2507535122-1-e2507535122-10 (2025)

A novel inhibitor against the bromodomain protein 1 of the malaria pathogen Plasmodium falciparum

Amann M., Warstat R., Rechten K.K., Theuer P., Schustereder M., Clavey S., Breit B., Einsle O., Hügle M., Petter M., Günther S.,
ChemMedChem 20, e202500024-1-e202500024-5 (2025)

Targeting pregnane X receptor with a potent agonist-based PROTAC to delay colon cancer relapse

Bansard L., Laconde G., Delfosse V., Huet T., Ayeul M., Rigal E., Donati Q., Gerbal-Chaloin S., Daujat-Chavanieu M., Brunel L., Legrand B., Chavanieu A., Martin A.R., Pannequin J., Bourguet W., Amblard M., Pascussi J.M.,
Oncogenesis 14, 34-1-34-15 (2025)

Altering substrate specificity of a thermostable bacterial monoamine oxidase by structure-based mutagenesis

Basile L., Poli C., Santema L.L., Lesenciuc R.C., Fraaije M.W., Binda C.,
Archives of Biochemistry and Biophysics 764, 110276-1-110276-7 (2025)

A fucose-binding superlectin from Enterobacter cloacae with high Lewis and ABO blood group antigen specificity

Beshr G., Sikandar A., Gläser J., Fares M., Sommer R., Wagner S., Köhnke J., Titz A.,
Journal of Biological Chemistry 301, 108151-1-108151-17 (2025)

Substitutions at the C-8 position of quinazolin-4-ones improve the potency of nicotinamide site binding tankyrase inhibitors

Bosetti C., Kampasis D., Brinch S.A., Galera-Prat A., Karelou M., Dhakar S.S., Alaviuhkola J., Waaler J., Lehtiö L., Kostakis I.K.,
European Journal of Medicinal Chemistry 288, 117397-1-117397-18 (2025)