The Protein Support Labs comprise various protein/biochemistry equipment available in several laboratories onsite that can provide support for experiments conducted on beamlines at the ESRF. The list of available equipment is shown below.

Users who wish to use any of the listed equipment during or for the preparation of an ESRF experiment must

  • Tick the "Protein Support Labs" box in the experiment session A-form, AND

  • Complete and send the Protein Support Labs Form to the Local Contact by email at least 15 days before the start date of the experiment. The form allows users to indicate the equipment needed, and the Local Contact (LC) information (name, email address) is available on the A-Form.

According to your needs and the availability of the equipment, you will be redirected to the appropriate laboratory(ies).

Please note that access to some of the labs needs to be planned in advance and will be allowed only during working hours (Monday to Friday, from 8am to 5pm). For every user, a specific on-site safety and building introduction (about 30 min long) will be required before accessing the lab.

 

List of available equipment

Available equipment
Analytical balance
Centrifuge eppendorfs, refrigerated
Centrifuge eppendorfs, non-refrigerated
Microscope OLYMPUS (100x)
Heating block (up to 95°C)
Freezer -80°C
Fridge / freezer
Ultrapure Water
Filtration degas set
Ice machine
Vortex
Magnetic stirring plate
UV/VIS spectrophotometry - Implen N60
Set of micropipettes

 

SEC columns (only available for SEC-SAXS experiments on BM29)

Column type ID Separation range (DA) Bed volume Sample volume Pressure limit Default flow rate
Superdex 200 Increase 10/300 GL ID0094 BM29 10K to 600K 24 mL 25-500 µL 2.6 MPa 0.5 mL/min
Superdex 75 10/300 GL ID0013 BM29 3K to 70K 25 mL 25-250 µL 1.8 MPa 0.5 mL/min
Superose 6 Increase 10/300 GL ID0041 BM29 5K to 5M 24 mL 25-500 µL 2.6 MPa 0.5 mL/min

 

On Demand equipment 
(only working days Mon-Fri from 8 am to 5pm. A specific on-site safety building training (about 30 min long) is mandatory before accessing).

Equipment details Further information
Titration - pHmeter Electrode InLab Expert Pro
Electrophoresis Biorad casting system with homemade gels
Beckmann Optima MAX 130K ultracentrifuge (rotors MLA-80, TLA-55) Users need to bring their own tubes
AKTA chromatography system @ RT Users need to bring their own columns
AKTA chromatography system (cold cabinet) Users need to bring their own columns
Bacterial culture shaking incubator -
Crystallisation room Users need to bring their own loops and cryotools