Chakraborty S., Alam B., Biswas S.,
Biochemical and Biophysical Research Communications 590, 145-151 (2022)
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ID30A-3 / MASSIF-3
Synopsis
Fixed energy microfocus beamline
Status:
open
Disciplines
- Life Sciences
Applications
- MASSIF-3 is a microfocus beamline (15 µm diameter), operating at fixed energy (12.81 keV). The beamline is dedicated to protein crystals of small size, inhomogeneous protein crystals, and fast data collection, and is also suitable for SAD measurements (Se, Pt, Au,...). MASSIF-3 is equipped with a MD2 micro-diffractometer and with a FlexHCD sample changer for SPINE pucks and UniPucks. Diffraction data are recorded on a Eiger X 4M detector that allows data collection at a very high frame rate (up to 750 Hz).
Techniques
- Anomalous diffraction
- MX - macromolecular crystallography
- Microbeam
- Microcrystallography
- Optical spectroscopy
- Single-crystal diffraction
- XRD - X-ray diffraction
Energy range
- 12.81 - 12.81 keV
Beam size
- Minimum (H x V) : 15.0 x 15.0 µm²
- Maximum (H x V) : 15.0 x 15.0 µm²
Sample environments
- 100 K cryostream
- automatic sample changer
- dehydration device
- online microspec
Detectors
- Eiger X 4M
References:
ID30A-3 (MASSIF-3) – a beamline for macromolecular crystallography at the ESRF with a small intense beam, D. Von Stetten et al., J. Synchrotron Radiat. (2020) 27: 844–851. doi: 10.1107/S1600577520004002
The Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation, P. Theveneau et al. (2013) J. Phys.: Conf. Ser. 425 012001 doi:10.1088/1742-6596/425/1/012001
Latest publications
View all publications
Katiki M., Neetu N., Pratap S., Kumar P.,
Biochimie 198, 8-22 (2022)
Kaya C., Walter I., Yahiaoui S., Sikandar A., Alhayek A., Konstantinovic J., Kany A.M., Haupenthal J., Köhnke J., Hartmann R.W., Hirsch A.K.H.,
Angewandte Chemie International Edition 61, e202112295-1-e202112295-5 (2022)
Liu B., Chan H., Bauda E., Contreras-Martel C., Bellard L., Villard A.M., Mas C., Neumann E., Fenel D., Favier A., Serrano M., Henriques A.O., Rodrigues C.D.A., Morlot C.,
Journal of Structural Biology 214, 107813-1-107813-11 (2022)
Matilla M.A., Velando F., Tajuelo A., Martin-Mora D., Xu W., Sourjik V., Gavira J.A., Krell T.,
mBio , epub (2022)
Pääkkönen J., Hakulinen N., Andberg M., Koivula A., Rouvinen J.,
Protein Science 31, 371-383 (2022)
partners
European Synchrotron Radiation Facility - 71, avenue des Martyrs, CS 40220, 38043 Grenoble Cedex 9, France.