Video How-tos

Video Synopsis of usage:

• Defining where your pucks are in the dewar (usually done by the local contact) /files/{workspace}/users/providers/ldap/gf/fi/fi/nanao/files/id23_images/define_dewar_position_from_shipment.mp4
• Defining Molecular Replacement search models and ligands /files/{workspace}/users/providers/ldap/gf/fi/fi/nanao/files/id23_images/EXI_proteins_ligands.mp4
• Logging in, synching MXcube3, characterisation and data collection https://www.esrf.fr/files/default/users/providers/ldap/gf/fi/fi/nanao/files/id23_images/Mxcube3%20Login%20Getcontrol%20Synch%20Char%20Collect-1.m4v
• X-ray Centering /files/{workspace}/users/providers/ldap/gf/fi/fi/nanao/files/id23_images/MxCuBE_XRC.mp4
• Helical data collection /files/{workspace}/users/providers/ldap/gf/fi/fi/nanao/files/id23_images/MxCuBE-Helical.mp4
• Connecting using NX and restarting the MXcube server /files/{workspace}/users/providers/ldap/gf/fi/fi/nanao/files/id23_images/NoMachine_restart_mxcube.mp4

??? Software overview:

• ISPYB: Laboratory Information Management System (LIMS) linking individual crystals to their corresponding X-ray data.
• MxCuBE: Graphical User Interface (GUI) for the beamline control.

Prepare your experiment:

• How to create a shipment form via the file upload in ISPYB

• (optional) Define PDBs for molecular replacement and possibly ligand files: /files/{workspace}/users/providers/ldap/gf/fi/fi/nanao/files/id23_images/EXI_proteins_ligands.mp4

• How to track your dewar
• How to see your sample information in MXCuBE - what you need to do in ISPYB

Running your experiment

• Defining where your pucks are in the dewar (usually done by the local contact) /files/{workspace}/users/providers/ldap/gf/fi/fi/nanao/files/id23_images/define_dewar_position_from_shipment.mp4
• Logging in, synching MXcube3, characterisation and data collection /files/{workspace}/users/providers/ldap/gf/fi/fi/nanao/files/id23_images/MXCuBE3_login_getcontrol_synch_char_collect_v2.mp4
• X-ray Centering /files/{workspace}/users/providers/ldap/gf/fi/fi/nanao/files/id23_images/MxCuBE_XRC.mp4
• Helical data collection /files/{workspace}/users/providers/ldap/gf/fi/fi/nanao/files/id23_images/MxCuBE-Helical.mp4
• Connecting using NX and restarting the MXcube server /files/{workspace}/users/providers/ldap/gf/fi/fi/nanao/files/id23_images/NoMachine_restart_mxcube.mp4