Video How-tos

Video Synopsis of usage:

• Defining where your pucks are in the dewar (usually done by the local contact)
• Defining Molecular Replacement search models and ligands
  • Alphafold IDs for molecular replacement
• Logging in, synching MXcube3, characterisation and data collection
• X-ray Centering
• Helical data collection
• Connecting using NX and restarting the MXcube server

??? Software overview:

• ISPYB: Laboratory Information Management System (LIMS) linking individual crystals to their corresponding X-ray data.
• MxCuBE: Graphical User Interface (GUI) for the beamline control.

Prepare your experiment:

• How to create a shipment form via the file upload in ISPYB

• (optional) Define PDBs for molecular replacement and possibly ligand files

• How to track your dewar
• How to see your sample information in MXCuBE - what you need to do in ISPYB

Running your experiment

• Defining where your pucks are in the dewar (usually done by the local contact)
• Logging in, synching MXcube3, characterisation and data collection
• X-ray Centering
• Helical data collection
• Connecting using NX and restarting the MXcube server