High throughput screening of frozen crystal

A Flex-HCD robotized sample changer, equipped with a dual gripper allows rapid mounting of frozen crystals on the goniometer. The storage Dewar can host 18 Unipucks. Vials have to be « Mitegen » or « Molecular Dimension » types.

Data collection at fixed wavelength

A MD2 goniometer is available for data collection on frozen crystals or crystals in capillaries.

Anomalous diffraction experiments

Selection of the wavelength between 6 to 20 keV

X-ray spectrometer

X-ray fluorescence spectrum can me measured with a Roentec X-Flash multi channel analyzer. This is required for the proper choice of wavelengths during an anomalous diffraction experiment, but can be used also for the detection of metal atoms in your protein.

On demand

On-line UV-Vis absorption spectroscopy

A UV-vis micro-spectrophotometer is available on demand for absorption measurements coupled to diffraction experiments.

Humidity controlled experiments

Humidity controlled air flow is available on request.

Services available off-line

Crystalization with the Opticrys bench

OptiCrys is a crystal growth apparatus for Optimization of protein Crystallization. It consists in the crystal growth bench that, in addition to accurate temperature control (with a temperature range of 233K-353K), also allows chemical composition of the crystallization solution (e.g. precipitant concentration, pH, additive) to be varied in an automated manner. OptiCrys enables monitoring and controlling the crystallization processes that allow producing crystals of specific sizes and morphology optimized for downstream structure determination.

Opticrys is available under a collaborative mode. Please, contact beamline staff if interested.