Fixed energy microfocus beamline
Status:  open


  • Life Sciences


  • MASSIF-3 is a microfocus beamline (15 µm diameter), operating at fixed energy (12.81 keV). The beamline is dedicated to protein crystals of small size, inhomogeneous protein crystals, and fast data collection, and is also suitable for SAD measurements (Se, Pt, Au,...). MASSIF-3 is equipped with a MD2 micro-diffractometer and with a FlexHCD sample changer for SPINE pucks and UniPucks. Diffraction data are recorded on a Eiger X 4M detector that allows data collection at a very high frame rate (up to 750 Hz).


  • Anomalous diffraction
  • MX - macromolecular crystallography
  • Microbeam
  • Microcrystallography
  • Optical spectroscopy
  • Single-crystal diffraction
  • XRD - X-ray diffraction

Energy range

  • 12.81 - 12.81  keV

Beam size

  • Minimum (H x V) : 15.0 x 15.0  µm²
  • Maximum (H x V) : 15.0 x 15.0  µm²

Sample environments

  • 100 K cryostream
  • automatic sample changer
  • dehydration device
  • online microspec


  • Eiger X 4M



 ID30A-3 (MASSIF-3) – a beamline for macromolecular crystallography at the ESRF with a small intense beam, D. Von Stetten et al., J. Synchrotron Radiat. (2020) 27: 844–851. doi: 10.1107/S1600577520004002


 The Upgrade Programme for the Structural Biology beamlines at the European Synchrotron Radiation Facility – High throughput sample evaluation and automation, P. Theveneau et al. (2013) J. Phys.: Conf. Ser. 425 012001 doi:10.1088/1742-6596/425/1/012001