A novel portable, high-luminosity monochromatically tuneable X-ray microscope. F. Flora1 ,S.Bollanti1, A. Lai1, P.Di Lazzaro1, T. Letardi1, A. Grilli2, L.Palladino3, G. Tomassetti3, A. Reale3, L. Reale3, A. Scafati3, L. Bacchetta4, L.Alianelli5, M.Sanchez del Rio6, T.A. Pikuz7 , A.Ya. Faenov7 1 ENEA, Dipartimento Innovazione, Settore Fisica Applicata, 00044 Frascati, Italy 2 INFN Frascati, 00044 Frascati, Italy 3 INFM Dipartamento di Fisica dell'Aquila and LNGS-INFN, Assergi (L'Aquila), Italy 4 Agriculture biotechnology Department, ENEA C.R. Casaccia, Via Anguillarese 301, 00060, Roma 5 INFM-OGG c/o ESRF, BP 220, F-38043 Grenoble Cedex, France, and ILL, BP 156, F-38042 Grenoble Cedex 6 European Synchrotron Radiation Facility, BP 220, F-38043 Grenoble Cedex, France 7 Multicharged Ions Spectra Data Center of VNIIFTRI, Mendeleevo, Moscow region, 141570 Russia Abstract. A novel experimental setup for transmission x-ray microscopy is presented. It is based on the use of a point isotrope x-ray source and a single spherical crystal. The x-ray beam intensity is modulated by the object attenuation, then monochromatized and enlarged using a spherical crystal and, lastly, imaged using a detector downstream of the crystal. We demonstrate by ray tracing technique and experimental testing that this system allows microscopy studies with image resolution better than the dimensions of the source, high magnification ratios, and great field of view. Microscopes using this model can be easily built using different micro x-ray sources, like conventional x-ray tube generators, x-rays emitted by laser generated plasmas or synchrotron radiation. Utilization of spherically bent crystals to obtain high-resolution, large field, monochromatic images in a wide range of Bragg angles (20°