Synopsis
The BioSAXS beamline is a highly automated beamline dedicated to the study of proteins, macromolecular complexes, viruses etc., in solution. Samples can be investigated under various conditions (temperature, buffer, pH, kinetics) in a high-throughput manner or a HPLC unit can be used for in-situ (online) purification.
Status:
open
Disciplines
- Life Sciences
- Chemistry
- Medicine
Applications
- Structural biology
- Pharmaceuticals
Techniques
-
BioSAXS - small-angle X-ray scattering (proteins/DNA)
-
SAXS - small-angle X-ray scattering
Beam size
- Minimum (H x V) : 50.0
x 50.0
µm²
-
Maximum (H x V) : 2.0
x 1.0
mm²
Sample environments
- Quartz capillary as a part of automated sample changer allowing temperature variations (from 4 to 60°C)
- HPLC (high performance liquid chromatography) system can be used in parallel with sample changer
Detectors
Pernot P., Theveneau P., Giraud T., Nogueira Fernandes R., Nurizzo D., Spruce D., Surr J., McSweeney S., Round A., Felisaz F., Foedinger L., Gobbo A., Huet J., Villard C. and Cipriani F., "New beamline dedicated to solution scattering from biological macromolecules at the ESRF", Journal of Physics: Conference Series 247 (2010) 012009-1-012009-8.
Studies of the structural properties and stability of the molybdenum transport protein ModA from Oleidesulfovibrio alaskensis G20 upon metal binding
Rivas M.G., Correia Cordeiro R.S., Nair R.R., Albuquerque L.J.C., Correia M.A.S., Panjkovich A., Svergun D.I., Santos-Silva T.,
Archives of Biochemistry and Biophysics 775, 110642-1-110642-11 (2026)
Dissection of an anaerobic fungal cellulosomal endoglucanase: Impact of the dockerin module on activity, thermostability, and flexibility
Andrade V.B., Tramontina R., Almeida D.V., Tomazetto G., da Silva V.M., Gabel F., Marcello Y.M.B., Scott A.L., Squina F.M., Garcia W.,
ACS Omega 10, 20474-20486 (2025)
HP1 loses its chromatin clustering and phase separation function across evolution
Bensaha S., Lewandowska D., Muzzopappa F., Hutin S., Tully M.D., Anfossi M., Cammas F.M., Normand C., Erdel F.,
Nature Communications 16, 6375-1-6375-15 (2025)
An interdisciplinary approach provides insights into the pronounced selectivity of compound 42 for DPP9
Beyens O., Corthaut S., Lambeir A., Van der Veken P., Sterckx Y.G.J., De Meester I., De Winter H.,
ChemMedChem 20, e202400700-1-e202400700-12 (2025)
Capabilities of SEC-SAXS method in research of polydisperse systems: The case of apoferritin
Bezruchko I.O., Kuklina D.D., Dronova E.A., Kazantsev A.S., Khokhlov A.A., Vlasov A.V., Kuklin A.I., Ryzhykau Y.L.,
Biochemistry (Moscow), Supplement Series A: Membrane and Cell Biology 19, 20-26 (2025)
Rabies virus phosphoprotein exhibits thermoresponsive phase separation with a lower critical solution temperature
Bouchama F., Mubashira K., Mas C., Le Roy A., Ebel C., Bourhis J.M., Zemb T., Prévost S., Jamin M.,
Journal of Molecular Biology 437, 168889-1-168889-25 (2025)
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